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Fig. S5 Cell death detection in the pLL of sox21a morphant embryos. A) Cell death was assayed by performing an immunostaining against cleaved Caspase 3 (red; left images) in control embryos (above images) and sox21a morphant embryos (bottom images). Right images show the overlay with the nuclear marker DAPI (blue). White dotted lines mark the borders of the pLL primordium and arrows point to cleaved Caspase 3 stained cells within the pLL primordium. B) The percentage of pLL primordia stained with cleaved Caspase 3 was quantified in Controls (Control; n=5), sox21a morpholino (MOsox21a; n=15), double sox21a and p53 morpholino (MOsox21a+MOp53; n=10) and SU5402 treated (SU5402; n=8) backgrounds.