Figures for Casari et al., 2014


Figure Caption/Comments:

Fig. 7 In vivo blocking of Smad3/TGFbeta; signaling impairs neuronal differentiation during early embryonic development. Confocal Z-stack tail images of 24 hpf transgenic embryos expressing GFP under control of neuronal promoter: ngn1, neuroD, and mnx1. Embryos were injected with morpholinos for smad3a at 1–2 cell stage or treated at 2 hpf with Alk5-inhibitor LY364947. As shown with an arrowhead, morpholinos and drug treatment give similar results: increase of proliferative cells (ngn1) at tail tip, decrease of differentiating cells (neuroD) and reduction of late differentiating motor neurons (mnx1). ngn1 is Tg(ngn1:GFP)sb1. neuroD is Tg(2.4 kb neurod:EGFP). mnx1 is Tg(hlxb9:GFP)ml2. Quantification of each frame is shown in Supplemental Table S3. Scale bar is 100 µm in all the images.

Figure Data:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.