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Fig. 6 EGFP-HSP70 zebrafish exposed to tBHQ A. Embryos from hsp70-EGFP transgenic zebrafish were exposed to DMSO (0.1%) or tBHQ (10 µM) for 4 hrs at 1-dpf. After exposure, embryos were washed and held for 4 additional hours prior to fluorescence microscopy for detecting EGFP expression. B. Another experiment was performed by exposing 4-dpf eleutheroembryos (n = 15) to DMSO (0.1%) or tBHQ (10 µM) for 4 hrs at 28°C. After exposure, eleutheroembryos were washed with 0.3× Danieau′s and inspected 4 hrs post-exposure for EGFP expression by fluorescence microscopy.
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