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Fig. 5

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ZDB-IMAGE-141229-3
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Figures for Yan et al., 2002
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Fig. 5 Morpholino antisense oligonucleotides directed against sox9a inhibit splicing and transport of sox9a transcript. (A) RNase protection assays of sox9a transcript in 28 hpf wild-type animals injected at the one-cell stage with 2 nl of different concentrations of MO, normalized against odc. (B) RNase protection assays of sox9a transcript in animals 28 hpf or 4 dpf either treated (+) or not treated (–) with 5.0 ng MO, normalized against odc. Graphs show the amount of RNA present as a percent of untreated controls. (C) Transcript for sox9a accumulates in the cytoplasm in wild type, but in the nucleus in splice-directed MO injected embryos at 28 hpf. In jeftw37 embryos, transcript is visible both in the cytoplasm and in the nucleus. Dorsal views, anterior is upwards. Equal amounts of MO directed against the donor sites for intron 1 and 2. Scale bars: 100 μm.

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