ZFIN Image: Chapman et al., 2014, Fig. S4

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Figure Caption

Fig. S4

Fibronectin is not required for homogeneous WM226-4 cell invasion and collagen I deposition is independent of fibronectin. (Related to Figure 5.)

(A) Wholemount immunofluorescence labelling of fibronectin in homogeneous xenografts of control (upper panel) and shFN#1 WM266-4 cells (lower panel). Mean ± SEM; unpaired Student’s T-test; N ≥ 15 from 3 independent experiments. (B) Wholemount immunofluorescence labelling of fibronectin and collagen I in homogeneous xenografts of control (top panel) and shFN#1 (bottom panel) WM266-4 cells. Scale bars = 100 μm. (C) Western blot showing fibronectin and collagen I expression in control and shFN#1 WM266-4 cells. (D) Relative invasion quantification of (A).

Acknowledgments

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