ZFIN Image: Chapman et al., 2014, Fig. 4

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Figure Caption

Fig. 4

ECM Proteins Correlate with Invasiveness

(A) Expression of ECM components collagen I and fibronectin in engrafted zebrafish 4 dpi; arrows indicate direction of invasion.

(B) Western blot showing collagen I and fibronectin expression in WM266-4 and 501mel cells.

(C) Collagen I (upper) and fibronectin (lower) in homogeneous compared to heterogeneous xenografts that are further treated with either DMSO or GM6001.

(D) Invasive WM266-4 and 501mel follow collagen I (upper) and fibronectin (lower) tracks radiating out from the tumor.

(E) Quantitation of ECM association. Cells were scored as being in touch “on” with collagen I or fibronectin strands or not “off.” Mean ± SEM; unpaired Student’s t test (collagen) and Mann-Whitney test (fibronectin); p < 0.0001; n e 18 from three independent experiments.

Acknowledgments

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