Fig. S3

BIO rescues expression of Wnt targets in dntcf3+ and dkk1+ embryos. (A–F) Whole mount ISH for gfp (A–C) and axin2 (D–F) at 30 hpf in embryos treated with DMSO, 50 µM BIO, and 100 µM BIO for 6 hours at 24 hpf. With increasing concentrations of BIO, 7xTCF:GFP embryos show an increase in ectopic gfp expression, particularly throughout the brain (B,C), Similarly, axin2 expression expands in a dose-dependent manner (E,F). (G–H) qPCR analysis for Wnt targets lef1 (G) and axin2 (H) in control, dntcf3+, and dkk1+ embryos treated with DMSO, 50 µM BIO, and 100 µM BIO for 6 hours at 2 hphs. Red line indicates normalized expression levels in DMSO treated non-transgenic controls with ef1alpha as an internal control. BIO induces expression of Wnt targets in non-transgenic controls and rescues expression in dntcf3+ and dkk1+ embryos (G,H).

Acknowledgments

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