Fig. 2

Fig. 2

sparse-like (kitlga^tc244b) caudal fins fail to regenerate melanocytes during the initial 7 days post amputation (dpa).

A, B, C. Wild-type, sparse-like (kitlga^tc244b), and sparse (kita^b5) fins were amputated and allowed to regenerate at 25°C. A′, B′, C′. No new melanocytes are observed during the first 3 dpa, irrespective of genotype. A′′. By 5 dpa, wild-type fins have newly differentiated kit-dependent melanocytes (grey arrowheads) in the regenerate. A′′′. By 7 dpa, more melanocytes emerge and organize into stripes. B′′-B′′′. kitlga^tc244b fins fail to produce new melanocytes during the first 7 dpa. C′′-C′′′. kita^b5 fins fail to produce new melanocytes during the first 7 dpa. The few melanocytes near the amputation plane (dashed-line) in the regenerate of kitlga^tc244b and kita^b5 fins are previously differentiated melanocytes (black arrowheads) that are drawn from the stump into the regenerated tissue as it grows. Scale bar = 500 mm.