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Fig. S5
The median fin fold defects in dlx5a/6a morphants are associated with altered cell proliferation and apoptosis. Lateral view at the posterior axis of BrdU (A, A2) and TUNEL (B, B2) assays on control (A–B) and dlx5a/6a morphant (A2–B2) embryos at 24 hpf. The BrdU assay shows that controls present proliferating cells in the median fin fold whereas no BrdU-positive cells are observed in the morphants. In parallel, the morphants show a high increase of apoptotic cells in the MFF compared to controls (B–B2). Scale bar shown in B2 for all panels 20 μm.
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