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Fig. S1
Images of larger field of view are used to demonstrate the general patterns and individual variability of huORFZ embryos treated with different heavy. (A) huORFZ embryos were treated with embryo media, ddH2O, or DMSO for 24 and 48 hr starting at 72 hpf. (B-D) The effects of different treatment times (24 and 48 hr) with different concentrations of cadmium (0.5, 1, and 5 μM), copper (1, 2 and 3 μM) and chlorpyrifos (4.2, 4.7, and 5.83 μM) on huORFZ embryos. For chlorpyrifos treated group, at 48 hr treatments are 100% lethal. All images were taken under the Leica MZ FLIII microscope with 2x objective. All images were taken under the same exposure time, iso value and other camera settings.