ZFIN Image: Dong et al., 2014, Fig. 4

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Figure Caption

Fig. 4

The PI3K/mTOR pathway contributes to the expansion of definitive myelopoiesis in pten-/- embryos. (A-L) Whole-mount in situ hybridization analyses of lyz-positive myeloid cells in embryos treated with increasing doses of LY294002 (LY) or rapamycin (Ra) at 90 hpf. Untreated and mock-treated pten-/- embryos (B, C and H, I) showing expansive myelopoiesis compared to the controls (A and G), as mentioned above. The pten-/- embryos treated with stepwise increases in LY294002 or rapamycin showed an obvious rescue effect, as indicated by the decrease in myeloid cells (D-F and I-L). The red arrowheads indicate the main positions of the myeloid cells. (M and N) Quantification of myeloid cells in each embryo (a-l). The data shown are the means ± SEM of at least 30 embryos, *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding controls. Scale bar: 100 μm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.