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Fig. 7
MCH intestinal upregulation in TNBS-induced enterocolitis in adult zebrafish.
A) Relative mRNA expression of the two different MCH peptides MCH1 and MCH2 in the zebrafish intestine following 6 hours of TNBS intrarectal infusion. TBP serves as the housekeeping gene. (n=9-10 fish per group). For each gene, expression in the vehicle treated group has been set to 100. AU: arbitrary mRNA units.
B) Immunofluorescence analysis of MCH positive cells in the zebrafish intestine at baseline and in response to TNBS treatment. An antibody against human MCH was used for staining zebrafish MCH.
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