ZFIN Image: Rengarajan et al., 2014, Fig. 6

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Figure Caption

Fig. 6

Inhibition of Fgf8 transport from early to late endosome does not alter signaling range.

Analysis of localization of Fgf8-GFP during inhibition of endocytic transport from early to late endosomes by treatment with Bafilomycin A1. Embryos were injected with Fgf8-GFP along with the early endosomal marker Rab5 or membrane marker mCherry at one cell stage, treated with 100 nM Bafilomycin A1 at 30% epiboly stage for a period of 1 hr and subjected to live imaging using confocal microscopy at 50% epiboly stage. At 75% epiboly stage embryos were fixed and stained for erm expression by ISH. Circle highlight co-localization of Rab5 and Fgf8, white arrows point to typical Fgf8 localizations, and balck arrows visualize the extend of the pea3 expression domain.

Acknowledgments

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