ZFIN Image: Rengarajan et al., 2014, Fig. 1

Image

Figure Caption

Fig. 1

Hsc70 regulates the Fgf8 signaling range.

(A–L) In situ hybridization for Fgf target genes erm and pea3 at 75% epiboly stage. Embryos were mounted laterally with animal pole to the top and dorsal region towards the left. The area of the expression domains of Fgf target genes erm and pea3 were investigated in embryos injected with indicated constructs and the border of the expression domains were marked by arrows. (E, F, K, L) show embryos co-treated with 16 μM of the Fgf signaling inhibitor SU5402. Fluorescent ISH for target gene expression pea3 is shown in (M–O). As an in-vivo reporter for Fgf signaling, the expression of Dusp6-EGFP in live zebrafish embryos at 50% epiboly stage was investigated (P–R). The area of expression of target genes erm and pea3 (A–L) was quantified in 10 embryos for each experiment (S).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One