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Fig. 4

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ZDB-IMAGE-140731-31
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Figures for George et al., 2014
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Fig. 4

Dark adaptation increases the number of vesicular structures in nrca14 photoreceptor inner segments.

TEM images of cone photoreceptors in light (A, B) or dark-adapted (C, D) WT and nrca14 retinas. At 4 dpf, larvae were phenotyped by OKR and placed at 28°C either on a normal light/dark cycle, or in complete darkness. 24 hours later, at 5 dpf, larvae were fixed for TEM. Dark adaptation exaggerated the vesicular structure phenotype in nrca14 cone photoreceptor inner segments (B vs. D). Cells were scored as “normal”, “vesiculated” or “severely vesiculated” and the quantification is shown in E. Cells with at least 3 vesicular structures >125 nm were scored as “vesiculated” and examples are shown by arrow heads in B and D, cells that contained at least 20 vesicular structures were scored as “severely vesiculated” and an example is shown by an arrow in D. OS = outer segment, N = nuclei. Scale bar = 2 μm in A–D. Graph shows mean, error bars are STDEV for three independent light/dark experiments. In total at least 400–500 cells from 5 larvae were counted per light condition and genotype.

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