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Fig. 2
The Gal4-ERT system provides rapid induction of gene expression.
(A–D) Kinetics of EGFP expression in Tg(krt5:Gal4-ERT-VP16; UAS:EGFP) animals upon administration of 2 μM 4-OHT at 24 hpf for 0–4.5 hours. (A2–D2) High magnification images demonstrating EGFP expression for each treatment. White arrowheads point to epidermal expression of EGFP; blue arrows indicate bleed-through from the heart muscle-specific ECFP transgenesis marker.
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