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Fig. S9
Genetic inactivation of Duox1 using a DN form partially prevents neutrophil infiltration into the skin of Tnfa- and Tnfr2-deficient zebrafish. Zebrafish one-cell mpx:eGFP embryos were injected with standard control (Std), Tnfr2, or Tnfa MOs alone or combination with antisense (As) or DN-Duox1 mRNAs. Representative images of bright field and green channels of morphants at 72 hpf showing the differences in the neutrophils distribution (A) and quantification of neutrophil mobilization from the CHT to the skin in the indicated number of larvae per group from three different experiments (B). The mean ± S.E.M. for each group is shown. Scale bars, 100 μm. ns, not significant. ***p<0.001.