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Fig. S6
Macrophage ablation is inducible and systemic in Tg(mpeg1:NTR-YFP) fish. (A) Macrophages are easily visualized throughout the pectoral fin and ocular area in Tg(mpeg1:NTR-eYFP) fish and addition of MTZ (for 2d) visually decreases macrophage number. Scale bar = 60 μm. (B) Macrophage ablation is reversible by MTZ washout. Incubation of transgenic fish in MTZ for 36 h ablates most macrophages, and subsequent incubation in non-drug fish water for >2d results in gradual restoration of overall numbers of macrophages throughout the fish, including the caudal fin. (C) Close-up images of mepg1+ and YFP+ cells in Tg(mpeg1:mCherry) and Tg(mpeg1:NTR-eYFP) fish in the caudal fin. Scale bar = 20 μm.