Fig. S3 Effect of Kiss1-SAP on Kiss1 immunoreactivity 3-d after injection. (A–D) Transverse sections of Kiss1 immunoreactivity in the ventral habenula (vHb; A and B) and the median raphe (MR; C and D) in the brain of zebrafish injected with Blank-SAP (A and C) or Kiss1-SAP (B and D) at 3 d after injection (103 mol per fish, intracranial administration; n = 3 per group). (Scale bars, 10 µm.) Note that there was no effect of Kiss1-SAP on Kiss1-immunoreactivity compared with in Blank-SAP treated fish at 3-d after injection, which could be due to a time course effect. Therefore, we decided to observe the effect of Kiss1-SAP at 12-d after injection (Fig. 6).
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