ZFIN Image: Nishiya et al., 2014, Fig. 5

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Figure Caption

Fig. 5

GGTI-286 Inhibits Nuclear Accumulation of ß-catenin

(A) GGTI-286 diminished ß-catenin nuclear accumulation. CHO cells were pretreated with 10µm GGTI-286 for 2 or 4 hr, treated with 2µm BIO for 2 hr, and then mechanically lysed and fractionated. The nuclear (Nuc) or cytoplasmic (Cyto) fractions of ß-catenin were analyzed by Western blotting with an antibody against ß-catenin. An anti-actin antibody was used for the loading control.

(B) GGTI-286 reduced Wnt3A-induced nuclear accumulation of ß-catenin. MDA-MB-231 cells were pretreated with 30µm GGTI-286 for 4 hr, treated with 50 ng/ml Wnt3A for 18 hr, and then stained with an anti-ß-catenin antibody, Hoechst33342, and phalloidin. Fluorescent images were observed under a confocal microscope. Scale bars represent 10µm.

Acknowledgments

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