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Fig. S1

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ZDB-IMAGE-140515-42
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Figures for Sun et al., 2014
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Fig. S1

Controls for mgaMOs used in this study, related to Figure 2. (A) Western blot from extracts of 75% epiboly mgaMO>1-cell morphants, probed with anti- MgaI or β-tubulin antibodies. TLMO1 Upper band: 6% ± 0.7 S.D. of control levels, Lower band: 30% ± 3.5 S.D. of control levels (not shown); TLMO2 Upper band: 28% ± 4.2 S.D. of control levels, Lower band: 39% ± 2.1 S.D. of control levels; TLMO3 Upper band: is 5% ± 0.7 S.D. of control levels, short isoform is 29% ± 1.4 S.D. of control levels (not shown). (A′) Western blot from extracts of 75% epiboly wildtype embryos, probed with anti-MgaN or anti-MgaC -­6-­ antibodies. The 250Kd and 290Kd bands are apparent in both lanes. (B, C, D, E, H, I) Image of live embryos at the prim-5 stage (24 hpf), injected at the 1-cell stage with 4ng each of mgamisMOTL3 and p53MO or mgaMOTL3 and p53MO (C, D), or injected into the YSL with 150pg IgG or anti-Max antibody (D, E, H, I). (F, G) eve1 expression in mgamisMO>YSL or mgaMO>YSL morphants. (J) Frequency of embryos with defects shown in panel (C) (red) in mgaTL1/3MO>1-cell morphants or mgaTL3MO>1-cell morphants injected with 50pg mRNA encoding either β-galactosidase or mouse mga mRNA. (K) Frequency of embryos lacking ventral tailfins (red) in mgaTL1MO>YSL morphants or mgaTL3MO>YSL morphants injected either with 50pg mRNA encoding β-galactosidase or mouse mga mRNA, or with 50pg DNA encoding zebrafish mga expressed under control of the CMV promoter or empty vector. Note that the pCS2+ zebrafish mga construct lacks sequences targeted by mgaTLMO3 but contains sequences targeted by mgaTLMO1. The mouse mga mRNA is not recognized by either MO. *pd0.05, **pd0.005, ***pd0.0005.

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Reprinted from Developmental Cell, 28(3), Sun, Y., Tseng, W.C., Fan, X., Ball, R., and Dougan, S.T., Extraembryonic signals under the control of MGA, Max, and Smad4 are required for dorsoventral patterning, 322-334, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell