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Fig. 3
Morpholino knockdown of dusp27 phenocopies y241 mutants. (A) Validation of morpholino efficacy for dusp27 knockdown using a dusp27 N-terminal sequence fused to GFP. (B) Morphology of embryos injected with control morpholino (top panel) or 1 ng of morpholino against dusp27 (bottom panel). Embryos show pronounced edema (asterisk). (C) Spontaneous coiling in embryos injected with control morpholino (black, n=11), 1 ng dusp27 morpholino (blue, n=9) or 2 ng dusp27 morpholino (gray, n=9). Inset, average coiling during 24–28 hpf for the same larvae. *P<0.01. Graphs show mean ± s.e.m.
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