Image
|
Figure Caption
Fig. S3
Injection procedure to measure the distance of Fgf8 distribution. Figure shows the schematic diagram of the experiment performed to analyze the spreading of Fgf8 from its source. (A) Fgf8 GFP DNA was injected into live zebrafish embryos at the one cell stage. (B) Zebrafish embryo at 50% epiboly stage show a mosaic expression of Fgf8. (C) Confocal microscopy analysis of live embryos show Fgf8 expressed in specific cells creating local sources from which the distance travelled by Fgf8 from the source to receiving cells is measured (e.g. a,b).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ PLoS One