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Fig. S1
A. Immunoblot analysis of the expression of Hsc70 protein during knockdown and over expression of Hsc70. Embryos were injected with Hsc70 morpholinos as well as Hsc70 mRNA and samples at 28hpf were subjected to immunoblot analysis. Embryos injected with Hsc70 MOs showed a reduction in the expression of Hsc70 when compared to the expression in wild type samples. Embryos overexpressing Hsc70 showed an increase in the expression of Hsc70 when compared to the expression in the wild type. ISH for Fgf8 expression. (B) Shows the expression of Fgf8 at 75% epiboly stage. Fgf8 expression remains unaltered during overexpression (C), knock-down of Hsc70 (D), and injection of Hsc70 MO and mRNA (E).