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Fig. 4
Ethanol exposure significantly increased the number of TUNEL-labeled cells in the O2 neuromast.
Apoptotic activity in O2 neuromasts was measured by TUNEL labeling of untreated and ethanol-treated Brn3c-GFP larvae from 2-5 dpf. (A, D) Untreated controls showed few TUNEL-labeled cells but (B, D) 1% ethanol-treated and (C, D) 1.5% ethanol-treated larvae showed a significant increase in the number of TUNEL-labeled cells (arrowhead) versus control animals. While there were more TUNEL-labeled cells in ethanol-treated animals when compared to untreated controls, there was no statistical difference between ethanol concentrations. Results are the mean values ± SD. n = 9-12 neuromasts for each treatment group. *p<0.05 and **p<0.01 when compared to untreated controls.