ZFIN Image: Johnson et al., 2014, Fig. 3

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Figure Caption

Fig. 3 Loss of Kif11 causes monoaster spindle formation in radial glial cells during division. kif11 siblings and wild type embryos treated with either STLC or DMSO control from 5 to 30 hpf were labeled for radial glia (Gfap), microtubules (α-Tubulin) (microtubules), and histones (Hoechst stain) to identify mitotic spindle phenotypes. (A) kif11 wild type siblings and (I) vehicle control (DMSO) treated embryos exhibit normal spindles in M-phase (enlarged in B–D and J–L, respectively) while (E) kif11^-/- mutants and (M) STLC treated embryos display monoastral spindles characteristic of mitotic arrest (enlarged in F–H and N–P, respectively). White dots designate presumptive locations of centrosomes. For greater clarity Hoechst is displayed as a greyscale image in the enlargements (D, H, L, P).

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Acknowledgments

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Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.