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Fig. S1

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ZDB-IMAGE-140411-2
Source
Figures for Xu et al., 2013
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Figure Caption

Fig. S1

Myogenesis Suppressors Identified In Vitro also Affect Muscle Development In Vivo, Related to Figure 1

(A) Higher magnification of myf5-GFP;mylz2-mCherry blastomere cells cultured in zESC medium with bFGF. Scale bar represents 50 μm.

(B) myf5-GFP;mylz2-mCherry embryos injected with 400pg con-MO or co-injected with 200pg myf5-MO and 200pg myoD-MO lack both myf5-GFP and mylz2-mCherry expression. Images taken at 30 hpf. Scale bars represent 200 μm.

(C) myf5-GFP;mylz2-mCherry embryos injected with 400pg con-MO or co-injected with 200pg myf5-MO and 200pg myoD-MO were disassociated at the oblong stage and cultured in zESC medium with bFGF. No myf5-GFP or mylz2-mCherry expression was detected in the culture of double morphants. Images were taken 26 hr after plating. Scale bars represent 200 μm.

Acknowledgments
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Reprinted from Cell, 155(4), Xu, C., Tabebordbar, M., Iovino, S., Ciarlo, C., Liu, J., Castiglioni, A., Price, E., Liu, M., Barton, E.R., Kahn, C.R., Wagers, A.J., and Zon, L.I., A Zebrafish Embryo Culture System Defines Factors that Promote Vertebrate Myogenesis across Species, 909-921, Copyright (2013) with permission from Elsevier. Full text @ Cell