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Fig. 1 Two-color double in situ hybridization identifies overlapping asymmetric gene expression patterns in the heart field (A-D) and diencephalon (E-J) of 22- to 24- somite stage zebrafish embryos. Ventral views, anterior at top, of lft2 expression (in red) and nkx2.5 (A), bmp4 (B), cyc (C) or pitx2 (D) expression (in purple), in the heart field. (E-J) Coexpression of lft1 (in red) with cyc (E-G, in purple) and pitx2 (H-J, in purple) in the left diencephalon (E, H, lateral views, dorsal at right; F, I, dorsal views, anterior at top; G, J, transverse sections, dorsal at top).
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