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Fig. 5 CA-BRIA ventralizes mesoderm like BMP ligand. (a) A schematic drawing of wild-type and constitutively active form of BMPR-IA. The glutamine (Q) residue located at position 228 was replaced with aspartate (D) in the constitutively active variant. (b) Dorsal views (A,B,E,F) and dorsoanimal views (C,D) of experimental (left column) and b-galactosidase mRNA-injected (right column) embryos. All embryos are oriented with their animal pole up. Suppression of ntl (A) and gsc (C) expression in the embryos injected with mRNA encoding CA-BRIA revealed the absence of axial mesoderm. By contrast, expansion of wnt-8 to the dorsal part demonstrated the mesoderm ventralization instead of dorsal mesoderm. Scale bar, 200 μm.