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Fig. 3 (A-C) Nkx2.5 expression can be detected at the onset of gastrulation. (A) It is restricted to the ventral margin of the embryo at 50% epiboly. The yolk is scrapped off intentionally to visualize the gradient of Nkx2.5 and the arrowheads point to the edge of the embryo. B shows goosecoid expression (arrowhead) as a dorsal reference. (C) The Nkx2.5- expressing cells migrate in the hypoblast, the term used to refer to deeper cell layers in zebrafish gastrulation. e, epiblast; h, hypoblast; y, yolk; An, animal pole; D, dorsal.
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