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Fig. 5

ID
ZDB-IMAGE-140228-23
Source
Figures for Sagerström et al., 1996
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Figure Caption

Fig. 5 Ventral marginal zone explants suppress the expression of otx2 in animal caps. (A) Schematic representation of dissections and conjugate preparation. Animal caps from sphere stage embryos (late blastula, 4 hpf) were aggregated into groups of 10 and either cultured alone or conjugated to ventral marginal zone (VMZ) explants from shield stage embryos (early gastrula, 6 hpf). Each conjugate contained 5 animal caps and 1 VMZ explant. Animal caps and conjugates were harvested when control embryos reached the 12 somite stage (15 hpf, 9 hours of culture). D, dorsal; V,ventral. (B) Whole-mount in situ hybridization analysis of otx2 expression. Animal caps (a) and conjugates (b) were analyzed for otx2 expression after 9 hours in culture. The VMZ lineage label (see Materials and Methods) is visualized in light blue and animal caps are unlabelled. This data is included in Table 3.

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