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Fig. 1
Expanded G4C2 Repeats Form Intranuclear RNA Foci
(A–D) SH-SY5Y cells were transfected with various length G4C2 (0, 8, 38, 72 repeat) plasmids and analyzed 24 hr after transfection by RNA FISH using a Cy3-labeled (G2C4)×8 RNA probe.
(E) The mean number of G4C2 foci was counted in 50 cells.
(F–I) Primary hippocampal mouse neurons (F), CV1 (G), HEK293 (H), and HeLa (I) cells were transfected with a plasmid expressing G4C2 72× repeats; foci were detected by FISH (red) and nuclei were stained with DAPI (blue). All cell types transfected with 38× and 72× repeats showed foci (scale bar represents 3 μm).
See also Figure S1