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Fig. 5 Onset of glomerular filtration. (A) Wild-type embryos injected with rhodamine dextran (10,000 Mr) into the heart at 48 hpf show extensive accumulation of dye in interstitium and in basement membranes but not in the lumen of the gut (g). Dye is present in the lumen of the pronephric duct indicating that the pronephric filtration is functioning at this time. By 56 hpf (B), capillary invaginations in the glomerulus appear more extensive and dye can found in vesicles in the apical cytoplasm of the pronephric ducts. da, dorsal aorta; gl, glomerulus; g, gut; pd, pronephric duct.
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