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Fig. 5
Endodermal pouch-specific knock-down of tbx2a causes an anomaly of the pharyngeal arches rescued by tbx2a mRNA.
(A) Taram-A* (tar*) mRNA injected into the marginal blastomere at the 16-cell stage gives rise to mesendoderm, from which the endodermal pouches derive. (B) Co-injection with MO2 affects the posterior pharyngeal arches (crosses). (C) Alcian Blue staining viewed under bright field microscope. Confocal imaging of the endodermal pouches upon co-injection with fluorescent dye and tar* mRNA in the (D) control and (E) the morphants co-injected with MO2. These morphants exhibit shortened and thickened endodermal pouches. (F) Western blot of total lysates from c-myc-tagged tbx2a mRNA-injected embryos (lane 1, 20 μg; lane 2, 100 μg) and non-injected embryos (lane 1′, 20 μg; lane 2′, 100 μg). (G) Rescued MO2-injected morphants with tbx2a mRNA show elongated endodermal pouches.