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Fig. 8 nkx2.5 and nkx2.7 work together to maintain vmhc expression and to limit amhc expression. (A-I) Fluorescence in situ hybridization depicts expression patterns of amhc (green) and vmhc (red) in wild-type (A-C), nkx2.5 mutant (D-F) and nkx2.5-/-;nkx2.7-/- double mutant (G-I) hearts at 52 hpf. Compared with robust expression of vmhc in both the wild-type (B) and nkx2.5 mutant (E) hearts, encroachment of ectopic amhc expression is demonstrated alongside dwindling vmhc expression in the ventricular remnant of the nkx2.5-/-;nkx2.7-/- mutant heart (G-I).
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