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Fig. S4
Expression of irx4a is progressively downregulated with increased loss of Nkx gene dosage.
(A-C) In situ hybridization depicts expression of irx4a in wild-type (A), nkx2.5 mutant (B), and nkx2.5-/-;nkx2.7-/- double mutant (C) embryos. Dorsal views, anterior to the top, at 26 hpf. Ventricular expression of irx4a (arrows) is diminished in nkx2.5 mutants and absent in nkx2.5-/-;nkx2.7-/- double mutants. Expression of irx4a is also visible in bilateral neurons of the lateral hindbrain.
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