Fig. 7

Fig. 7 Floor plate cilia are largely dispensable for FBM neuron migration. (A–H) Dorsal views of hindbrains with anterior to the left. The ratio of the number of embryos exhibiting a phenotype to the total number of embryos examined is indicated in each panel. (A,B) Anti-tubulin immunostaining (green). In a wildtype hindbrain (A), floor plate cilia (arrow) are uniformly orientated along the anterior-posterior axis, whereas in a vangl2 mutant (tri/) mutant (B), the floor plate cilia (arrow) are disorganized and misorientated. (C,D) Tg(isl1:GFP) embryos processed for anti-tubulin (red) and anti-GFP (green) immunostaining. In a wildtype sibling (C), floor plate cilia (arrow) are uniformly orientated, and FBM neurons (arrowhead) migrate out of r4. In an iguana/ embryo (D), although floor plate cilia are absent, FBM neurons (arrowhead) migrate normally out of r4. (E,F) While floor plate cilia (white arrow) are present and uniformly orientated in the Movl hindbrain (E), they are absent in the MZovl hindbrain (F). Prominent longitudinal axon tracts appear unaffected in MZovl embryos. (G,H) Tag1 in situs to label branchiomotor and sensory neurons. FBM neurons (white arrowheads) migrate out of r4 in both Movl and MZovl embryos, although a small number of neurons (black arrowhead) consistently remained in r4 in MZovl embryos. Asterisks indicate the location of sensory ganglia, which develop normally in both genotypes.