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Fig. 5 Comparison between patterns of UAS-driven mRFP fluorescence and endogenous gene expression by whole mount in situ hybridization. Embryos containing ebf3tpl16, cyp26c1tpl24 and snap25btpl27 gene traps and Tg(UAS:mRFP)tpl2 reporter where photographed on Zeiss AxioImager microscope at 30–32 hpf (A, C, and E respectively). Expression of endogenous genes detected by whole mount in situ hybridization using antisense probes against ebf3(B), cyp26c1(D) and snap25b(F) on 30 hpf embryos.
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