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Fig. S4

ID
ZDB-IMAGE-131107-24
Source
Figures for Nachtrab et al., 2013
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Figure Caption

Fig. S4

Anterior transcription factor overexpression has little or no effect on pectoral fin regeneration. (A) Expression of alx4a, id4 or lhx9 is induced in posterior pectoral fin regions at 10-fold or higher levels in new transgenic lines, 4 hours after a single heat-shock at 4 dpa. Values are normalized to actb1 levels and relative to wild-type controls. n=3; **P<0.005, Student’s t-test; mean ± s.e.m. (B) Appearance of transgenic and wild-type clutchmate fins at 7 dpa after a series of daily heat shocks. Transgenic fins appear similar to wild-type fins. (C) Quantification of regenerate lengths indicates a minor change in some rays after anterior gene overexpression. n=12-16; *P<0.05, Student’s t-test. (D) Quantification of width ratios indicates a minor change in some rays. n=12-16; *P<0.05, Student’s t-test. Scale bars: 0.5 mm.

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