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Fig. S4 Atx-Lpar3 axis mediates gut looping. Embryos injected with 5 ng Std MO, lpar1 MO, lpar3 MO or atx MO were incubated until 48 hpf, fixed and subjected to WISH against foxA3. (A) Three representative gut looping phenotypes, including L-loop gut liver bud (Left), defected midline gut (Midline) and reversed R-loop gut (Reverse), were shown by WISH against foxA3 and insulin (ins). Insets showed corresponding cardiac looping patterns. (B) The percentages of each phenotype in all treatments are shown (n=3). Total number of embryos used in each treatment is shown on top of each column.
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