ZFIN Image: Powell et al., 2013, Fig. S8

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Figure Caption

Fig. S8

prdm1a dominant activator rescues foxd3 and tfap2a in prdm1a mutant embryos, and both activator and repressor forms rescue sox10. (A-H) Whole-mount ISH was performed on prdm1a^–/– embryos injected with prdm1aDBD-VP16 activator or prdm1a DBD-EnR repressor at 2-somites for foxd3 (A-D, dorsal views) and tfap2a (E-H, lateral views). foxd3 and tfap2a are both decreased in prdm1a^–/– embryos (B,F) compared with WT/het embryos (A,E). Injection of prdm1aDBD-VP16 partially rescues foxd3 (C) and expands tfap2a (G) at the NPB in prdm1a mutants, whereas prdm1aDBD-EnR does not rescue either foxd3 or tfap2a mRNA expression. (I-M) ISH for sox10 was performed on prdm1a^–/– embryos injected with prdm1aDBD-VP16, prdm1aDBD-EnR or both combined at 4-somites. prdm1aDBD-VP16 (K), prdm1aDBD-EnR (L) and both combined (M) partially rescued sox10 expression in the NPB. Dorsal views.

Acknowledgments

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