|
Fig. 4
tfap2a mRNA rescues prdm1a-deficient neural crest. (A-F) Uninjected WT zebrafish embryos (A,D), prdm1a morphants (B,E) and prdm1a morphants co-injected with tfap2a mRNA (C,F) were subject to ISH for the neural crest markers foxd3 at 2-somites (A-C) and sox10 at 4-somites (D-F). WT embryos show defined expression of foxd3 and sox10 at the NPB, which is significantly decreased in prdm1a morphants. tfap2a mRNA injection rescues the neural crest in prdm1a-deficient embryos. Dorsal views, anterior to the top. (G) The percentage of embryos expressing each marker. foxd3: WT, n=49/58; prdm1a-MO, n=13/120; rescue, n=27/72. sox10: WT, n=45/56; prdm1a-MO, n=3/62; rescue, n=37/76. *P<0.05. Error bars indicate s.e.m.