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Fig. 1

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ZDB-IMAGE-131015-39
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Figures for Xia et al., 2013
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Fig. 1 Phenotype analysis and proviral insertion mapping of zebrafish No. 12615 retrovirus insertional mutant. (A)–(D) Images of the phenotype of No. 12615 homozygous mutant embryos under bright-field. At 24 hpf, the mutant embryo (B) is indistinguishable from the wild-type (A); at 28 hpf, the mutant embryo (D) exhibits less pigmentation (red arrowhead) and slight edema of the pericardial cavity (blue arrowhead) than those of the wild-type (C). (E) eif3ba gene structure. Exogenous retroviral (RV) sequence (red box) inserted into the 2nd exon of eif3ba gene, and resulting in a pre-mature stop codon ‘TGA’. (F, F′) Predicted protein structure of Eif3ba, with part of the second and third exons encoding the important RNA-recognition motif (RRM) domain (orange bar) (F), while in the mutant, the ‘TGA’ stop codon in the proviral sequence leads to the production of a truncated protein which breaks the RRM domain at the second exon (F′). (G)–(I) The mutant embryos are largely rescued by injecting eif3ba mRNA. The injected mutant embryos (n=30/30) (I) show more pigmentation than the control (H) at 2 dpf, and the head morphology is more normal. (J)–(K′) The expression of crestin in the mutant embryos compared with the wild-type at 24 hpf. The expression of crestin dramatically decreased in the mutant embryos, specifically in the cranial region (red triangles) and the branchial primordia (red arrows). WT, wild type; Mut, eif3ba mutant. (A–D, G–I, J, K) Lateral view with anterior to the left and dorsal up; (J′, K′) dorsal view with anterior to the left.

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Reprinted from Developmental Biology, 381(1), Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S., and Zhang, B., Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish, 83-96, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.