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Fig. 2
Feeder cells expressing zebrafish Lif, Fgf2 and Gdnf promote spermatogonial cell proliferation for 3 weeks in culture.
Photomicrographs showing G418-selected spermatogonia that were initiated from Tg(piwil1:neo);Tg(piwil1:DsRed) zebrafish showing (A) 4-cell and (B) 15-cell colonies; DsRed expression in the (C) 4-cell and (D) 15-cell colonies. (E) OFC3LF and OFC3LG significantly enhanced spermatogonial cell proliferation in cultures maintained for 3 weeks while the mitogenic effect was lost after 6 weeks. * indicates a significant difference by Student t-tests. Scale bar = 20 μm.
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