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Fig. 6
Wortmannin and rapamycin phenocopy ethanol-treated pdgfra mutants. (A-L) 5 dpf zebrafish neurocrania embryos were treated at 10-24 hpf with (A-C) DMSO, (D-F) 1.0% ethanol, (G-I) 1.5 μM wortmannin or (J-L) 3 μM rapamycin. Asterisks indicate ethmoid-plate defects (ep); arrowheads indicate trabeculae defects (tr). (M) Graph of percentage defects found in all three genotypes, across treatments. Unaffected, wild-type phenotype; ep, ethmoid-plate defects (arrow and asterisk in K); tr, trabeculae defects (arrowheads in H).
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