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Fig. S9
Etv2 overexpression is toxic to angiogenic sprouts and its maintained expression prevents kdrl:GFP expression. (A) Time lapse imaging of an intersegmental vessel angiogenic sprout from Figure 3C showing apparent apoptosis. (B) Multiple heat shocks of hsp70l:etv2 embryos prevent ectopic kdrl:GFP expression. Embryos were initially heat shocked at 24 hpf and then either maintained at normal temperatures or were treated with heat shock every 12 h the indicated number of times. Embryos treated with multiple heat shocks displayed abnormal morphology whose severity correlated with the number of heat shocks, including cardiac edema suggestive of circulatory failure (left column and inset). Control embryos heat shocked four times appeared similar to non–heat shocked embryos, HS(–). When Etv2 expression was maintained for the whole period (4× heat shocks), kdrl:GFP was not ectopically induced and was reduced or absent in the normal vasculature (right column).