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Fig. S4
ISH of vascular genes following Etv2 expression. Fli1a (A), tal1 (B), erg (C), and kdr (D) are all induced in the trunk of embryos 8 h post–heat shock (HS+8 h). Normally these genes are specifically expressed in the vasculature at this time point (control), although tal1 is more strongly expressed in the blood and neurons in the CNS. Note that fli1a and erg have almost ubiquitous expression at this time point, while tal1 and kdr are more restricted to ectopic expression in the trunk. Quantification of the number of embryos demonstrating ectopic expression over the number observed is in the bottom right corner of the corresponding high-magnification trunk image for each group. Eight hours post–heat shock was chosen because it is the time when ectopic expression of kdrl:GFP was first noted in our transgenic analysis.