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Fig. S3 (related to Fig. 3) D4a receptor knock down reduces the number of different motor neuron classes. Lateral views at 33 hpf are shown. A: D4a knock down attenuates islet-1:GFP+ motor neuron development, which is not rescued by NPA (drug exposure 24 to 56 hpf; ANOVA, ***P < 0.0001). B: Numbers of HB9:GFP+ motor neurons are significantly reduced by morpholino treatment (24 to 33 hpf). C: Knock down of d4a had no effect on motor neuron numbers at segment 16-17, when TH1+ axons had only reached trunk segment 7. Error bars represent SEM. Scale bars: 50 μm.
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Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell