|
Fig. 4
The same locations on the retina can be recovered and viewed on subsequent days. Through a combination of approximate relocation using vessel branch patterns and more precise relocation using unique lesion edge patterns, areas of interest were reexamined at multiple time points. A: In the mCherry channel (blue-sensitive cones), a lesion edge reminiscent of a bay shape, and a single cone within the bay (arrow), was observed at 1 day post-lesion (DPL). Image in A is a merge of the green fluorescent protein (GFP) and mCherry channels; A′ shows the mCherry channel alone. B: The same bay-shaped lesion edge was relocated at 10 DPL. B is a merge of the GFP and mCherry channels; B′ shows a higher magnification of the white box in B, in the mCherry channel alone. The single cone (arrow) was also identified. The appearance of a new blue-sensitive cone between 1 DPL [A′] and 10 DPL [B′] is indicated with an empty arrow. The chevron indicates a potential surviving cone, but the faint signal from this location at 1 DPL puts to question if the cone died and was replaced by the bright cone seen at 10 DPL. C: An area containing uniquely-shaped patches of surviving ultraviolet-sensitive cones is observed at 8 DPL (top) and relocated at 10 DPL (bottom). The same collection of ultraviolet-sensitive cones, and the same individual cones, was identified (white boxes) at both days. D: An area of regenerated UV cones showing the loss of row mosaic organization, imaged at 35 and 42 DPL (panel D and D;′, respectively); matching clusters of cones are indicated with white boxes. For an example of the outlined vessels and how areas were located using vasculature, see Figure 3. n=2 fish shown.