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Fig. 4 Knockdown of lamb1a in the KV results in randomization of liver and pancreas asymmetry. (A-E) Confocal imaging of the gut in Tg(XlEef1a:GFP) embryos injected at 3 hpf (A) with control (B) or lamb1a (C-E) MO. Effect of KV knockdown of lamb1a on the L-R asymmetry of the liver (Li) and pancreas (Pa) was analyzed at 52 hpf. Resulting phenotypes of the liver and pancreas after KV-specific lamb1a knockdown include: wild type (C, blue); bilateral liver and pancreas and midline gut (D, red); inversion of L-R asymmetry, with the liver and pancreas localized to the right and left sides of the embryo, respectively (E, yellow). Ventral views. (F) Percentages (mean±s.e.m.) of embryos displaying distinct liver and pancreas L-R asymmetry phenotypes. Forty-two embryos were analyzed for each treatment. Asterisks indicate statistical significance, *P=0.001. (G) Percentages (mean±s.e.m.) of embryos showing distinct spaw expression patterns at 20 ss. Wild-type embryos were injected with control (n=28) or lamb1a (n=36) MO at 3 hpf.